Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

用于鉴定裂殖酵母多拷贝抑制因子的遗传筛选
 
Click here for the English version

用于鉴定裂殖酵母多拷贝抑制因子的遗传筛选

Article DOI: 10.3791/63967-v 13:22 min September 13th, 2022
September 13th, 2022

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

这项工作描述了 在Pombe裂殖酵母中进行多拷贝抑制遗传筛选的方案。该筛选使用全基因组质粒文库来鉴定与查询突变菌株相关的功能丧失表型的抑制克隆。使用该筛选鉴定 ell1 零突变体的新型遗传抑制因子。

Tags

生物学,第187期,遗传筛选,多拷贝抑制因子, 庞贝裂殖酵母,Ell1,基因调控,转录伸长
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter