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JoVE Journal Biology
Genetisk skærm til identifikation af multikopiundertrykkere i Schizosaccharomyces pombe
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Genetisk skærm til identifikation af multikopiundertrykkere i Schizosaccharomyces pombe

Article DOI: 10.3791/63967-v • 13:22 min • September 13th, 2022
September 13th, 2022 •
*1, *1, 1, 1
1University School of Biotechnology, G.G.S. Indraprastha University
* These authors contributed equally

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Dette arbejde beskriver en protokol for en multikopi suppressor genetisk skærm i Schizosaccharomyces pombe. Denne skærm bruger et genom-dækkende plasmidbibliotek til at identificere suppressorklon (er) af en funktionstabsfænotype forbundet med en forespørgselsmutantstamme. Nye genetiske suppressorer af ell1 null mutant blev identificeret ved hjælp af denne skærm.

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Biologi udgave 187 genetisk skærm multikopiundertrykkere Schizosaccharomyces pombe Ell1 genregulering transkriptionsforlængelse
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