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Rekonstituering av membranbundet minimal aktin cortices på støttede lipid dobbeltlag
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Rekonstituering av membranbundet minimal aktin cortices på støttede lipid dobbeltlag

Article DOI: 10.3791/63968-v • 11:55 min • July 12th, 2022
July 12th, 2022 •
1, 2, 2, 2
1Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, 2National Centre for Biological Sciences, Tata Institute of Fundamental Research

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Denne protokollen beskriver dannelsen av støttede lipid-dobbeltlag og tilsetning av cytoskeletale filamenter og motorproteiner for å studere dynamikken i rekonstituerte, membranbundne cytoskeletale nettverk ved bruk av fluorescensmikroskopi.

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