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JoVE Journal Neuroscience
Millisekund hydrogen / deuterium-utveksling massespektrometri for studiet av alfa-synuclein strukturell dynamikk under fysiologiske forhold
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Millisekund hydrogen / deuterium-utveksling massespektrometri for studiet av alfa-synuclein strukturell dynamikk under fysiologiske forhold

Article DOI: 10.3791/64050-v • 08:40 min • June 23rd, 2022
June 23rd, 2022 •
1, 1
1Living Systems Institute, Department of Biosciences, University of Exeter

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Det strukturelle ensemblet av monomer alfa-synuclein påvirker dets fysiologiske funksjon og fysisk-kjemiske egenskaper. Denne protokollen beskriver hvordan man utfører millisekund hydrogen / deuterium-utveksling massespektrometri og påfølgende dataanalyser for å bestemme konformasjonsinformasjon om monomeren til dette iboende uordnede proteinet under fysiologiske forhold.

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Nevrovitenskap utgave 184 Alfa-synuclein indre lidelse millisekund hydrogen / deuterium-utveksling massespektrometri dynamisk strukturbiologi
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