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Journal / Immunology and Infection / En strategi för studier av IL-9-producerande…
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Immunology and Infection

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JoVE Journal Immunology and Infection
En strategi för studier av IL-9-producerande lymfoida celler i Nippostrongylus brasiliensis-infektionsmodellen
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En strategi för studier av IL-9-producerande lymfoida celler i Nippostrongylus brasiliensis-infektionsmodellen

Article DOI: 10.3791/64075-v • 08:38 min • March 3rd, 2023
March 3rd, 2023 •
*1, *1, *1, 1, 1, 1
1Departamento de Biología Celular y del Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México
* These authors contributed equally

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IL-9-uttryckande T- och ILC2-celler induceras under N. brasiliensis-infektion , men deras karakterisering har till stor del förbisetts i den infekterade tarmen på grund av deras lågfrekventa och differentiella kinetik. Detta protokoll beskriver isoleringen av dessa celler från olika målorgan och bekräftelse av deras identitet via flödescytometri vid olika infektionsstadier.

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Immunologi och infektion IL-9 Th9 ILC2 IL-9 lymfoida celler parasit tunntarmen N. brasiliensis
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