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Journal / Immunology and Infection / En strategi for undersøgelse af IL-9-producerende …
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Immunology and Infection

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JoVE Journal Immunology and Infection
En strategi for undersøgelse af IL-9-producerende lymfoide celler i Nippostrongylus brasiliensis-infektionsmodellen
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En strategi for undersøgelse af IL-9-producerende lymfoide celler i Nippostrongylus brasiliensis-infektionsmodellen

Article DOI: 10.3791/64075-v • 08:38 min • March 3rd, 2023
March 3rd, 2023 •
*1, *1, *1, 1, 1, 1
1Departamento de Biología Celular y del Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México
* These authors contributed equally

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IL-9-ekspressive T- og ILC2-celler induceres under N. brasiliensis-infektion , men deres karakterisering er stort set blevet overset i den inficerede tarm på grund af deres lavfrekvente og differentielle kinetik. Denne protokol beskriver isoleringen af disse celler fra forskellige målorganer og bekræftelse af deres identitet via flowcytometri på forskellige infektionsstadier.

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Immunologi og infektion udgave 193 IL-9 Th9 ILC2 IL-9 lymfoide celler parasit tyndtarm N. brasiliensis
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