JoVE Journal
Neuroscience
Neuroscience
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MRI, 조직 투명화 및 라이트 시트 현미경을 사용한 온전한 신생아 마우스 뇌의 전뇌 단일 세포 이미징 및 분석
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Article
DOI:
10.3791/64096-v
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08:49 min
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August 1st, 2022
MRI, 조직 투명화 및 라이트 시트 현미경을 사용한 온전한 신생아 마우스 뇌의 전뇌 단일 세포 이미징 및 분석
August 1st, 2022
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Felix A. Kyere*1,2, Ian Curtin*1,2, Oleh Krupa1,2, Carolyn M. McCormick1,2, Mustafa Dere3, Sarah Khan3,7, Minjeong Kim7, Tzu-Wen Winnie Wang4,5,6, Qiuhong He4,5,6, Guorong Wu3, Yen-Yu Ian Shih4,5,6, Jason L. Stein1,2
1UNC Neuroscience Center, University of North Carolina, Chapel Hill, 2Department of Genetics, University of North Carolina, Chapel Hill, 3Department of Psychiatry, University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, 6Department of Neurology, The University of North Carolina at Chapel Hill, 7Department of Computer Science, The University of North Carolina at Greensboro
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
이 프로토콜은 iDISCO+를 사용하여 온전한 마우스 뇌의 자기 공명 영상, 제거 및 면역 표지를 수행하는 방법을 설명한 다음, 라이트 시트 현미경을 사용한 이미징 및 NuMorph를 사용한 다운스트림 분석에 대한 자세한 설명을 설명합니다.