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Journal / Biology / Dechiffrera högupplöst 3D-kromatinorganisation…
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JoVE Journal Biology
Dechiffrera högupplöst 3D-kromatinorganisation via Capture Hi-C
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Dechiffrera högupplöst 3D-kromatinorganisation via Capture Hi-C

Article DOI: 10.3791/64166-v • 09:32 min • October 14th, 2022
October 14th, 2022 •
1, 1, 2, 1, 3, 4, 1, 1,5
1EMBL: European Molecular Biology Laboratory, 2Institut Curie, 3Agilent Technologies, 4Genmab BV, 5Collège de France

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Detta protokoll beskriver Capture Hi-C-metoden som används för att karakterisera 3D-organisationen av megabaserade riktade genomiska regioner med hög upplösning, inklusive gränser för topologiskt associerande domäner (TADs) och långväga kromatininteraktioner mellan regulatoriska och andra DNA-sekvenselement.

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