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Immunology and Infection

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JoVE Journal Immunology and Infection
Trennung von Immunzellsubpopulationen in peripheren Blutproben von Kindern mit infektiöser Mononukleose
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Trennung von Immunzellsubpopulationen in peripheren Blutproben von Kindern mit infektiöser Mononukleose

Article DOI: 10.3791/64212-v • 08:44 min • September 7th, 2022
September 7th, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences

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Wir beschreiben eine Methode, die immunmagnetische Beads und fluoreszenzaktivierte Zellsortierung kombiniert, um definierte Immunzellsubpopulationen peripherer mononukleärer Blutzellen (Monozyten, CD4+ T-Zellen, CD8+ T-Zellen, B-Zellen und natürliche Killerzellen) zu isolieren und zu analysieren. Mit dieser Methode können magnetisch und fluoreszierend markierte Zellen gereinigt und analysiert werden.

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Immunologie und Infektion Ausgabe 187 Epstein-Barr-Virus (EBV) infektiöse Mononukleose (IM) immunmagnetische Perlensortierung fluoreszierend aktivierte Zellsortierung (FACS) Immunzellsubpopulationen
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