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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

정점 장내구 모델에서 루시퍼 옐로우를 사용한 장 투과성 측정

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Journal (Immunology and Infection)

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface

Journal (Immunology and Infection)

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface

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정점 장내구 모델에서 루시퍼 옐로우를 사용한 장 투과성 측정

Article DOI: 10.3791/64215-v • 09:48 min • July 27th, 2022

July 27th, 2022 •

Heather Liebe¹, Camille Schlegel², Xue Cai², Alena Golubkova¹, Tyler Leiva¹, William L. Berry³, Catherine J. Hunter¹

¹Division of Pediatric Surgery, Oklahoma Children's Hospital, ²Oklahoma University Health Sciences Center, ³Department of Surgery, University of Oklahoma Health Sciences Center

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본 프로토콜은 장 투과성을 결정하기 위해 정점 엔터로이드 모델에서 루시퍼 옐로우를 활용하는 방법을 간략하게 설명합니다. 이 방법은 괴사 성 장염과 같은 염증성 장 질환을 모델링하는 장내 세포에서 세포 투과성을 결정하는 데 사용할 수 있습니다.

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면역학 및 감염 문제 185

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