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Journal / Immunology and Infection / Bestemme intestinal permeabilitet ved hjelp av…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Bestemme intestinal permeabilitet ved hjelp av lucifer gul i en apikal-ut enteroid modell
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Bestemme intestinal permeabilitet ved hjelp av lucifer gul i en apikal-ut enteroid modell

Article DOI: 10.3791/64215-v • 09:48 min • July 27th, 2022
July 27th, 2022 •
1, 2, 2, 1, 1, 3, 1
1Division of Pediatric Surgery, Oklahoma Children's Hospital, 2Oklahoma University Health Sciences Center, 3Department of Surgery, University of Oklahoma Health Sciences Center

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Denne protokollen skisserer en metode som benytter lucifer gul i en apikal-ut enteroid modell for å bestemme intestinal permeabilitet. Denne metoden kan brukes til å bestemme paracellulær permeabilitet i enteroider som modellerer inflammatoriske tarmsykdommer som nekrotiserende enterocolitt.

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Immunologi og infeksjon utgave 185
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