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Journal / Biology / Kvantitativ bestemmelse av de novo…
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JoVE Journal Biology
Kvantitativ bestemmelse av de novo fettsyresyntese i brunt fettvev ved bruk av deuteriumoksid
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Kvantitativ bestemmelse av de novo fettsyresyntese i brunt fettvev ved bruk av deuteriumoksid

Article DOI: 10.3791/64219-v • 07:34 min • May 12th, 2023
May 12th, 2023 •
*1, *2, 1, 2,3,4
1UCD Conway Institute and UCD Institute of Food and Health, School of Agriculture and Food Science, University College Dublin, 2Division of Endocrinology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 3Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 4Department of Pediatrics, University of Cincinnati College of Medicine
* These authors contributed equally

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Her presenterer vi en billig kvantitativ metode som benytter deuteriumoksid og gasskromatografi massespektrometri (GCMS) for analyse av total fettsyre de novo lipogenese i brunt fettvev in vivo.

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Biologi utgave 195 brunt fettvev deuteriumoksid metabolsk vei funksjonelle roller helkroppens metabolske homeostase fysiologiske prosesser patologiske prosesser glukoseavhending funksjonell vurdering detaljerte protokoller kvantitativ metode gasskromatografi massespektrometri (GCMS) total fettsyresyntese karbonkilde prøvepreparering nedstrømsberegninger brunt fett metabolsk homeostase
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