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Journal / Biology / Kvantitativ bestämning av de novo-fettsyrasyntes…
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Kvantitativ bestämning av de novo-fettsyrasyntes i brun fettvävnad med hjälp av deuteriumoxid
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Kvantitativ bestämning av de novo-fettsyrasyntes i brun fettvävnad med hjälp av deuteriumoxid

Article DOI: 10.3791/64219-v • 07:34 min • May 12th, 2023
May 12th, 2023 •
*1, *2, 1, 2,3,4
1UCD Conway Institute and UCD Institute of Food and Health, School of Agriculture and Food Science, University College Dublin, 2Division of Endocrinology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 3Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 4Department of Pediatrics, University of Cincinnati College of Medicine
* These authors contributed equally

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Här presenterar vi en billig kvantitativ metod som använder deuteriumoxid och gaskromatografi masspektrometri (GCMS) för analys av total fettsyra de novo lipogenes i brun fettvävnad in vivo.

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Biologi utgåva 195 brun fettvävnad deuteriumoxid metabolisk väg funktionella roller metabolisk homeostas i hela kroppen fysiologiska processer patologiska processer glukosbortskaffande funktionsbedömning detaljerade protokoll kvantitativ metod gaskromatografimasspektrometri (GCMS) total fettsyra de novo-syntes kolkälla provberedning nedströmsberäkningar brunt fett metabolisk homeostas
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