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Journal / Genetics / Detectie van homologe recombinatietussenproducten …
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JoVE Journal Genetics
Detectie van homologe recombinatietussenproducten via nabijheidsligatie en kwantitatieve PCR in saccharomyces cerevisiae
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Detectie van homologe recombinatietussenproducten via nabijheidsligatie en kwantitatieve PCR in saccharomyces cerevisiae

Article DOI: 10.3791/64240-v • 07:55 min • September 11th, 2022
September 11th, 2022 •
1, 2, 2, 1,3
1Department of Microbiology & Molecular Genetics, University of California, Davis, 2Laboratory of Biology & Modeling of the Cell, École Normale Supérieure de Lyon, 3Department of Molecular & Cellular Biology, University of California, Davis

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De D-loop capture (DLC) en D-loop extension (DLE) assays maken gebruik van het principe van proximity ligatie samen met kwantitatieve PCR om D-loopvorming, D-loop extensie en productvorming te kwantificeren op de plaats van een induceerbare dubbelstrengs breuk in Saccharomyces cerevisiae.

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Genetica D-loop genoomstabiliteit gewrichtsmolecuul Rad51 break-induced replication
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