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JoVE Journal Genetics
Påvisning av homologe rekombinasjonsmellomprodukter via nærhetsligering og kvantitativ PCR i Saccharomyces cerevisiae
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Påvisning av homologe rekombinasjonsmellomprodukter via nærhetsligering og kvantitativ PCR i Saccharomyces cerevisiae

Article DOI: 10.3791/64240-v • 07:55 min • September 11th, 2022
September 11th, 2022 •
1, 2, 2, 1,3
1Department of Microbiology & Molecular Genetics, University of California, Davis, 2Laboratory of Biology & Modeling of the Cell, École Normale Supérieure de Lyon, 3Department of Molecular & Cellular Biology, University of California, Davis

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D-loop capture (DLC) og D-loop extension (DLE) analysene benytter prinsippet om nærhetsligering sammen med kvantitativ PCR for å kvantifisere D-loop-formasjon, D-loop-utvidelse og produktdannelse på stedet for et induserbart dobbeltstrenget brudd i Saccharomyces cerevisiae.

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Genetikk D-loop genomstabilitet leddmolekyl Rad51 break-indusert replikasjon
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