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Flowcytometrianalyse af murinknoglemarv, hæmatopoietisk stam- og stamceller og stromale nicheceller
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Flowcytometrianalyse af murinknoglemarv, hæmatopoietisk stam- og stamceller og stromale nicheceller

Article DOI: 10.3791/64248-v • 08:34 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1, 1, 1,2,3,4
1Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto, 2Department of Onco-Hematology, Instituto Português de Oncologia (IPO)-Porto, 3Unit of Biochemistry, Department of Biomedicine, Faculdade de Medicina da Universidade do Porto, 4Porto Comprehensive Cancer Center (P.CCC)

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Her beskriver vi en simpel protokol til isolering og farvning af murinknoglemarvsceller til fænotype hæmopoietiske stam- og stamceller sammen med de understøttende nicheendotel- og mesenkymale stamceller. En metode til at berige celler placeret i endosteale og centrale knoglemarvsområder er også inkluderet.

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Tilbagetrækning nr. 187
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