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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolamento e quantificazione del virus Epstein-Barr dalla linea cellulare P3HR1
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Isolamento e quantificazione del virus Epstein-Barr dalla linea cellulare P3HR1

Article DOI: 10.3791/64279-v • 09:14 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1,2
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, 2Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut

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Questo protocollo consente l'isolamento delle particelle virali di Epstein-Barr dalla linea cellulare umana P3HR1 inducendo il ciclo litico virale con forbolo 12-miristato 13-acetato. Il DNA viene successivamente estratto dalla preparazione virale e sottoposto a PCR in tempo reale per quantificare la concentrazione di particelle virali.

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Immunologia e infezione numero 187
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