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Journal / Immunology and Infection / P3HR1 세포주에서 엡스타인-바 바이러스의 분리 및…
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Immunology and Infection
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JoVE Journal Immunology and Infection
P3HR1 세포주에서 엡스타인-바 바이러스의 분리 및 정량화
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P3HR1 세포주에서 엡스타인-바 바이러스의 분리 및 정량화

Article DOI: 10.3791/64279-v • 09:14 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1,2
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, 2Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut

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이 프로토콜은 phorbol 12-myristate 13-아세테이트로 바이러스 용해 주기를 유도할 때 인간 P3HR1 세포주에서 Epstein-Barr 바이러스 입자의 분리를 허용합니다. 이어서 바이러스 제제에서 DNA를 추출하고 실시간 PCR을 수행하여 바이러스 입자 농도를 정량화합니다.

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면역학 및 감염 문제 187
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