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JoVE Journal
Biology

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结合透明和荧光显微镜,可视化基因表达的变化和对芸苔疟原虫的生理反应
 
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结合透明和荧光显微镜,可视化基因表达的变化和对芸苔疟原虫的生理反应

Article DOI: 10.3791/64297-v 06:58 min August 5th, 2022
August 5th, 2022

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Summary

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Click here for the English version.

本协议描述了一种优化的方法,用于组织学观察由 芸苔疟原虫 诱导的瘿。在荧光成像之前清除下胚轴的振动切片,以研究疾病进展过程中转录因子和植物激素的参与。该方案克服了树脂包埋的限制 ,使荧光 蛋白的植物可视化成为可能。

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生物学,第186期,
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