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JoVE Journal Cancer Research
Et flowcytometribaseret celleoverfladeproteinbindende assay til vurdering af selektivitet og specificitet af en anticancer aptamer
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Et flowcytometribaseret celleoverfladeproteinbindende assay til vurdering af selektivitet og specificitet af en anticancer aptamer

Article DOI: 10.3791/64304-v • 10:46 min • September 13th, 2022
September 13th, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1School of Medicine, Deakin University, 2Institute for Mental and Physical Health and Clinical Translation, School of Medicine, Deakin University

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Et nødvendigt skridt i udvikling af anticancer aptamer er at teste dets binding til målet. Vi demonstrerer et flowcytometrisk baseret assay for at studere denne binding og understreger vigtigheden af at inkludere en negativ kontrolaptamer og kræftceller, der er positive eller negative for det pågældende protein.

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