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Journal / Cancer Research / Avaliação da Saúde Mitocondrial em Fibroblastos…
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JoVE Journal Cancer Research
Avaliação da Saúde Mitocondrial em Fibroblastos Associados ao Câncer Isolados de Esferoides de Tumores Pulmonares Multicelulares 3D
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Avaliação da Saúde Mitocondrial em Fibroblastos Associados ao Câncer Isolados de Esferoides de Tumores Pulmonares Multicelulares 3D

Article DOI: 10.3791/64315-v • 10:26 min • October 21st, 2022
October 21st, 2022 •
*1, *1, 1, 1
1Department of Biomedical Engineering, Indian Institute of Technology Ropar
* These authors contributed equally

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Esferoides tumorais 3D multicelulares foram preparados com células de adenocarcinoma pulmonar, fibroblastos e monócitos, seguidos pelo isolamento de fibroblastos associados ao câncer (CAFs) desses esferoides. CAFs isolados foram comparados com fibroblastos normais para avaliar a saúde mitocondrial estudando o potencial transmembranar mitocondrial, espécies reativas de oxigênio e atividades enzimáticas.

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Cancer Research Edição 188 fibroblastos associados ao câncer CAFs esferoides tumorais multicelulares metabolismo mitocondrial espécies reativas de oxigênio ROS
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