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Cancer Research
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评估从3D多细胞肺肿瘤球体中分离的癌症相关成纤维细胞的线粒体健康
 
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评估从3D多细胞肺肿瘤球体中分离的癌症相关成纤维细胞的线粒体健康

Article DOI: 10.3791/64315-v 10:26 min October 21st, 2022
October 21st, 2022

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Summary

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Click here for the English version.

用肺腺癌细胞、成纤维细胞和单核细胞制备多细胞3D肿瘤球体,然后从这些球体中分离癌症相关成纤维细胞(CAF)。将分离的CAF与正常成纤维细胞进行比较,通过研究线粒体跨膜电位,活性氧和酶活性来评估线粒体健康状况。

Tags

癌症研究,第188期,癌症相关成纤维细胞,CAF,多细胞肿瘤球状体,线粒体代谢,活性氧,ROS
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