A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Please note that all translations are automatically generated.
用肺腺癌细胞、成纤维细胞和单核细胞制备多细胞3D肿瘤球体,然后从这些球体中分离癌症相关成纤维细胞(CAF)。将分离的CAF与正常成纤维细胞进行比较,通过研究线粒体跨膜电位,活性氧和酶活性来评估线粒体健康状况。