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Immunology and Infection

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JoVE Journal Immunology and Infection
Fagmedieret genmanipulation af borreliose Spirochete Borrelia burgdorferi
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Fagmedieret genmanipulation af borreliose Spirochete Borrelia burgdorferi

Article DOI: 10.3791/64408-v • 09:01 min • September 28th, 2022
September 28th, 2022 •
1
1Department of Biomedical Sciences, Quinnipiac University

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Bakteriofagens evne til at flytte DNA mellem bakterieceller gør dem til effektive værktøjer til genetisk manipulation af deres bakterielle værter. Præsenteret her er en metode til at inducere, genoprette og bruge φBB-1, en bakteriofag af Borrelia burgdorferi, til at transducere heterologt DNA mellem forskellige stammer af Lyme-sygdommen spirochete.

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Immunologi og infektion udgave 187 Borrelia burgdorferi bakteriofag vandret genoverførsel transduktion
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