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Journal / Immunology and Infection / Væsentlige komponenter i Borreliella (Borrelia)…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Væsentlige komponenter i Borreliella (Borrelia) burgdorferi In vitro transkriptionsassays
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Væsentlige komponenter i Borreliella (Borrelia) burgdorferi In vitro transkriptionsassays

Article DOI: 10.3791/64134-v • 07:15 min • July 22nd, 2022
July 22nd, 2022 •
1, 1, 1
1School of Medicine, Creighton University

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In vitro transkriptionsassays kan dechiffrere mekanismerne for transkriptionel regulering i Borreliella burgdorferi. Denne protokol beskriver trinene til oprensning af B. burgdorferi RNA-polymerase og udførelse af in vitro-transkriptionsreaktioner . Eksperimentelle tilgange ved anvendelse af in vitro transkriptionsassays kræver pålidelig oprensning og opbevaring af aktiv RNA-polymerase.

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Denne måned i JoVE udgave 185 Borrelia Borreliella in vitro transkription RNA-polymerase RpoD RpoC
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