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Bioengineering

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JoVE Journal Bioengineering
Una camera flessibile per l'imaging di cellule vive time-lapse con microscopia a diffusione Raman stimolata
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Una camera flessibile per l'imaging di cellule vive time-lapse con microscopia a diffusione Raman stimolata

Article DOI: 10.3791/64449-v • 07:40 min • August 31st, 2022
August 31st, 2022 •
1, 1
1Department of Biomedical Engineering, Binghamton University, State University of New York

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Riportiamo una camera ambientale flessibile per l'imaging time-lapse di cellule vive utilizzando la microscopia a diffusione Raman stimolata verticalmente con rilevamento del segnale trasmesso. Le goccioline lipidiche sono state visualizzate in cellule SKOV3 trattate con acido oleico per un massimo di 24 ore con un intervallo di tempo di 3 minuti.

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Retrazione Numero 186 Camera flessibile imaging di cellule vive time-lapse diffusione Raman stimolata microscopia goccioline lipidiche metabolismo lipidico del cancro
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