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Journal / Bioengineering / Time-lapse imaging di fluorescenza della crescita …
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Bioengineering
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JoVE Journal Bioengineering
Time-lapse imaging di fluorescenza della crescita Root Arabidopsis con manipolazione accelerato dell'ambiente root usando il RootChip
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Time-lapse imaging di fluorescenza della crescita Root Arabidopsis con manipolazione accelerato dell'ambiente root usando il RootChip

Article DOI: 10.3791/4290-v • 13:54 min • July 7th, 2012
July 7th, 2012 •
1, 2,3,4, 1, 1, 2,3, 1, 1
1Department of Plant Biology, Carnegie Institution for Science, 2Howard Hughes Medical Institute, 3Departments of Applied Physics and Bioengineering, Stanford University, 4Department of Microsystems Engineering (IMTEK) and Center for Biological Signaling Studies (BIOSS), University of Freiburg

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Questo articolo fornisce un protocollo per la coltivazione di piantine di Arabidopsis in RootChip, una piattaforma di imaging microfluidico che combina controllo automatizzato delle condizioni di crescita con il monitoraggio radice al microscopio e FRET-based misura i livelli di metaboliti intracellulari.

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Bioingegneria Biologia Vegetale Fisica Fisiologia Vegetale radici microfluidica imaging idroponica,
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