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Isolering av regenererende hepatocytter etter delvis hepatektomi hos mus
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Isolering av regenererende hepatocytter etter delvis hepatektomi hos mus

Article DOI: 10.3791/64493-v • 10:04 min • December 2nd, 2022
December 2nd, 2022 •
1, 1
1Swiss HPB Laboratory, Department of Visceral & Transplant Surgery, University Hospital Zurich (USZ)

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Lipidbelastede hepatocytter er iboende for leverregenerering, men går vanligvis tapt ved sentrifugering av tetthetsgradient. Her presenterer vi en optimalisert celleisolasjonsprotokoll som beholder steatotiske hepatocytter, noe som gir representative populasjoner av regenererende hepatocytter etter delvis hepatektomi hos mus.

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