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Isolering av regenererande hepatocyter efter partiell hepatektomi hos möss
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Isolering av regenererande hepatocyter efter partiell hepatektomi hos möss

Article DOI: 10.3791/64493-v • 10:04 min • December 2nd, 2022
December 2nd, 2022 •
1, 1
1Swiss HPB Laboratory, Department of Visceral & Transplant Surgery, University Hospital Zurich (USZ)

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Lipidbelastade hepatocyter är inneboende för leverregenerering men förloras vanligtvis vid densitetsgradientcentrifugering. Här presenterar vi ett optimerat cellisoleringsprotokoll som behåller steatotiska hepatocyter, vilket ger representativa populationer av regenererande hepatocyter efter partiell hepatektomi hos möss.

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