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Biochemistry

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JoVE Journal Biochemistry
En "dual-addisjon" kalsiumfluorescensanalyse for høy gjennomstrømningsscreening av rekombinante G-proteinkoblede reseptorer
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En "dual-addisjon" kalsiumfluorescensanalyse for høy gjennomstrømningsscreening av rekombinante G-proteinkoblede reseptorer

Article DOI: 10.3791/64505-v • 08:46 min • December 2nd, 2022
December 2nd, 2022 •
1, 2, 1
1Department of Entomology, Texas A&M University, 2Department of Biochemistry and Biophysics, Texas A&M University

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I dette arbeidet beskrives en intracellulær kalsiumfluorescensanalyse med høy gjennomstrømning for 384-brønnplater for å skjerme små molekylbiblioteker på rekombinante G-proteinkoblede reseptorer (GPCR). Målet, kininreseptoren fra storfefeberflåtten, Rhipicephalus microplus, uttrykkes i CHO-K1-celler. Denne analysen identifiserer agonister og antagonister som bruker de samme cellene i en "dual-addisjon" -analyse.

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