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Journal / Biochemistry / Imagerie de cellules vivantes de globes ex vivo…
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Biochemistry

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JoVE Journal Biochemistry
Imagerie de cellules vivantes de globes ex vivo intacts à l’aide d’un nouveau support imprimé en 3D
Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis
Journal (Biochemistry)
Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis
Biolayer Interferometry (BLI) for Evaluating PROTACS-Induced Ternary Complex Formation (Video) | JoVE
Journal (Biochemistry)
Biolayer Interferometry (BLI) for Evaluating PROTACS-Induced Ternary Complex Formation (Video) | JoVE
 
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Imagerie de cellules vivantes de globes ex vivo intacts à l’aide d’un nouveau support imprimé en 3D

Article DOI: 10.3791/64510-v • 05:41 min • October 6th, 2022
October 6th, 2022 •
*1, *2, 2, 3, 2,3
1Department of Pharmacology, School of Medicine, Boston University, 2Department of Biochemistry, School of Medicine, Boston University, 3Department of Ophthalmology, School of Medicine, Boston University
* These authors contributed equally

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Le présent travail décrit un nouveau protocole expérimental qui utilise un support imprimé en 3D pour permettre l’imagerie de cellules vivantes à haute résolution de globes énucléés. Grâce à ce protocole, l’activité de signalisation cellulaire du calcium dans l’épithélium cornéen blessé des globes ex vivo peut être observée en temps réel.

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Biochimie numéro 188
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