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Biochemistry

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JoVE Journal Biochemistry
Imaging di cellule vive di globi ex vivo intatti utilizzando un nuovo supporto stampato in 3D
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Imaging di cellule vive di globi ex vivo intatti utilizzando un nuovo supporto stampato in 3D

Article DOI: 10.3791/64510-v • 05:41 min • October 6th, 2022
October 6th, 2022 •
*1, *2, 2, 3, 2,3
1Department of Pharmacology, School of Medicine, Boston University, 2Department of Biochemistry, School of Medicine, Boston University, 3Department of Ophthalmology, School of Medicine, Boston University
* These authors contributed equally

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Il presente lavoro descrive un nuovo protocollo sperimentale che utilizza un supporto stampato in 3D per consentire l'imaging di cellule vive ad alta risoluzione di globi enucleati. Attraverso questo protocollo, l'attività di segnalazione del calcio cellulare nell'epitelio corneale ferito da globi ex vivo può essere osservata in tempo reale.

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Biochimica Numero 188
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