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Journal / Immunology and Infection / Analyse av T-cellereseptorindusert…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Analyse av T-cellereseptorindusert kalsiuminnstrømning i primære Murine T-celler ved fullspektret flowcytometri
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Analyse av T-cellereseptorindusert kalsiuminnstrømning i primære Murine T-celler ved fullspektret flowcytometri

Article DOI: 10.3791/64526-v • 10:01 min • December 16th, 2022
December 16th, 2022 •
1, 1, 1
1Department of Immunology and Microbiology, University of Colorado-Anschutz School of Medicine

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Kalsiuminnstrømning, et mål på T-cellesignalering, er en effektiv måte å analysere responser på T-cellereseptorstimulering. Denne protokollen for multipleksing av Indo-1 med paneler av antistoffer rettet mot celleoverflatemolekyler utnytter de svært fleksible egenskapene til fullspektret flowcytometri.

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Immunologi og infeksjon utgave 190
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