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Coltivazione, congelamento, lavorazione e imaging di interi organoidi e sferoidi mentre sono ancora in un idrogel
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Coltivazione, congelamento, lavorazione e imaging di interi organoidi e sferoidi mentre sono ancora in un idrogel

Article DOI: 10.3791/64563-v • 08:07 min • December 23rd, 2022
December 23rd, 2022 •
1,2, 2,3, 1, 3,5, 6, 2,3,4
1Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 2Cellorama, 3Cancer and Stem Cell Research Center, Maltepe University, 4Department of Histology and Embryology, School of Medicine, Maltepe University, 5Department of Medical Biology, School of Medicine, Maltepe University, 6School of Medicine, Koc University

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Il presente studio descrive le metodologie per la coltivazione, il congelamento, lo scongelamento, la lavorazione, la colorazione, l'etichettatura e l'esame di interi sferoidi e organoidi sotto vari microscopi, mentre rimangono intatti in un idrogel all'interno di un dispositivo multiuso.

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Biologia Numero 190
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