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Una piattaforma ad alto rendimento per la coltura e l'imaging 3D di organoidi
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Una piattaforma ad alto rendimento per la coltura e l'imaging 3D di organoidi

Article DOI: 10.3791/64405-v • 07:42 min • October 14th, 2022
October 14th, 2022 •
1,2, 1, 1, 1, 1, 1, 1,3,4, 1,5
1Mechanobiology Institute (MBI), National University of Singapore, 2Biomedical Engineering Department, National University of Singapore, 3Department of Biological Sciences, National University of Singapore, 4IRL 3639 CNRS, 5Department of Microbiology & Immunology, Immunology Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore

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Questo documento presenta un protocollo di fabbricazione per un nuovo tipo di substrato di coltura con centinaia di microcontenitori per mm2, in cui gli organoidi possono essere coltivati e osservati utilizzando la microscopia ad alta risoluzione. Anche i protocolli di semina cellulare e immunocolorazione sono dettagliati.

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Biologia Numero 188
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