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Journal / Cancer Research / 계대증 없이 정상 및 종양 유방 조직에서 마우스 및 인간 상피 오가노이드 생성 및…
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Cancer Research

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JoVE Journal Cancer Research
계대증 없이 정상 및 종양 유방 조직에서 마우스 및 인간 상피 오가노이드 생성 및 이미징
Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System
Journal (Cancer Research)
Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System
Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors
Journal (Cancer Research)
Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors
 
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계대증 없이 정상 및 종양 유방 조직에서 마우스 및 인간 상피 오가노이드 생성 및 이미징

Article DOI: 10.3791/64626-v • 08:57 min • November 11th, 2022
November 11th, 2022 •
1,2, 2,3,4,5, 1,2, 1,2, 1,2, 6, 1,2, 2,3,4,5, 1,2,3,4
1Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern Medical Center, 2Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 3Department of Molecular Biology, University of Texas Southwestern Medical Center, 4Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, 5Department of Cell Biology, University of Texas Southwestern Medical Center, 6Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California

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이 프로토콜은 차등 원심분리를 통해 원발성 정상 및 종양 유방 조직에서 상피 오가노이드를 생성하는 접근 방식에 대해 논의합니다. 또한 3차원 배양 및 포매된 오가노이드의 면역형광 이미징에 대한 지침이 포함되어 있습니다.

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