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JoVE Journal Cancer Research
Generera och avbilda mus- och humana epitelorganoider från normal och tumörbröstvävnad utan att passera
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Generera och avbilda mus- och humana epitelorganoider från normal och tumörbröstvävnad utan att passera

Article DOI: 10.3791/64626-v • 08:57 min • November 11th, 2022
November 11th, 2022 •
1,2, 2,3,4,5, 1,2, 1,2, 1,2, 6, 1,2, 2,3,4,5, 1,2,3,4
1Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern Medical Center, 2Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 3Department of Molecular Biology, University of Texas Southwestern Medical Center, 4Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, 5Department of Cell Biology, University of Texas Southwestern Medical Center, 6Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California

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Detta protokoll diskuterar ett tillvägagångssätt för att generera epitelorganoider från primär normal och tumörbröstvävnad genom differentiell centrifugering. Vidare ingår instruktioner för tredimensionell odling samt immunofluorescerande avbildning av inbäddade organoider.

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Cancerforskning nummer 189
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