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Vurdering af tarmbarriereintegritet hos mus ved hjælp af fluorescein-isothiocyanat-mærket dextran
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Vurdering af tarmbarriereintegritet hos mus ved hjælp af fluorescein-isothiocyanat-mærket dextran

Article DOI: 10.3791/64710-v • 05:14 min • November 18th, 2022
November 18th, 2022 •
1, 1,2, 1, 1,3
1Nutrition and Microbiome Laboratory, Institut du cancer de Montréal, Centre de recherche du Centre hospitalier de l’Université de Montréal (CRCHUM), 2Digestive Surgery Service, Centre hospitalier de l’Université de Montréal (CHUM), Faculty of Medicine, Université de Montréal, 3Department of Medicine, Faculty of Medicine, Université de Montréal

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I denne undersøgelse administreres fluorescein-isothiocyanatmærket (FITC) dextran til mus via oral sonde for at evaluere tarmpermeabilitet både in vivo og i plasma- og fækalprøver. Da tarmbarrierefunktionen påvirkes i mange sygdomsprocesser, kan dette direkte og kvantitative assay anvendes inden for forskellige forskningsområder.

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Biologi nr. 189
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