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Funktionel vurdering af intestinal tæt forbindelsesbarriere og ionpermeabilitet i indfødt væv ved ussing chamber teknik
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Funktionel vurdering af intestinal tæt forbindelsesbarriere og ionpermeabilitet i indfødt væv ved ussing chamber teknik

Article DOI: 10.3791/62468-v • 06:43 min • May 26th, 2021
May 26th, 2021 •
1, 1, 1
1Laboratory of Physiology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka

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Tarmepitel giver ikke kun næringsstofabsorption, men beskyttelse mod skadelige stoffer. Det apikaleste epitel intercellulære kryds, dvs. det stramme kryds, regulerer paracellulært opløst stof og ionpermeabilitet. Her beskrives en protokol til fremstilling af slimhindeplader og vurdering af ionselektiviteten af tætte kryds ved hjælp af Ussing-kammerteknik.

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Biologi udgave 171
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