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JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
CRISPR SunTag-p65-HSF1活性化系を用いたベージュ脂肪の生態と代謝の検討
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CRISPR SunTag-p65-HSF1活性化系を用いたベージュ脂肪の生態と代謝の検討

Article DOI: 10.3791/64849-v • 09:52 min • January 6th, 2023
January 6th, 2023 •
*1, *1, 1,2
1Obesity and Comorbidities Research Center, State University of Campinas, 2Department of Structural and Functional Biology, Institute of Biology, State University of Campinas
* These authors contributed equally

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このプロトコルは、ベージュ脂肪生物学を調査するためのアデノ随伴ウイルス(AAV)の代替戦略として、脂肪細胞(AdipoSPH)におけるCRISPR SunTag-p65-HSF1(SPH)の使用を示しています。内因性Prdm16遺伝子を標的とするAAV担持sgRNAの インビボ 注射は、ベージュ脂肪の発生を誘導し、熱発生遺伝子プログラムを増強するのに十分である。

Tags

今月のJoVE、第191号、脂肪組織、熱発生、代謝、CRISPR
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