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Journal / Genetics / 具有eGFP组成型表达的 利什曼原虫…
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Genetics
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JoVE Journal Genetics
具有eGFP组成型表达的 利什曼原虫 菌株的发展
High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo)
Journal (Genetics)
High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo)
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
Journal (Genetics)
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
 
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具有eGFP组成型表达的 利什曼原虫 菌株的发展

Article DOI: 10.3791/64939-v • 10:03 min • April 21st, 2023
April 21st, 2023 •
*1, *1, 1, 1, 2, 1,3, 1,3, 1,3
1Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), 2Departamento de Medios y Creativo, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), 3Sistema Nacional de Investigación-Secretaría Nacional de Ciencia, Tecnología e Innovación (SNI-SENACYT)
* These authors contributed equally

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在这里，我们描述了用于生成使用pLEXSY系统将eGFP基因表达为稳定整合转基因的L. panamensis 和 L. donovani 菌株的方法。通过限制稀释来克隆转染的寄生虫，并选择两种物种中荧光强度最高的克隆进一步用于药物筛选测定。

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遗传学，第194期，
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