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Journal / Genetics / CRISPR干扰（CRISPRI）在瘦素病原物种中基因沉默的应用…
JoVE Journal
Genetics
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JoVE Journal Genetics
CRISPR干扰（CRISPRI）在瘦素病原物种中基因沉默的应用
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CRISPR干扰（CRISPRI）在瘦素病原物种中基因沉默的应用

Article DOI: 10.3791/62631-v • 14:49 min • August 14th, 2021
August 14th, 2021 •
1,2, 1, *2, *1
1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2Laboratório de Desenvolvimento de Vacinas, Instituto Butantan
* These authors contributed equally

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在这里，描述了CRISPR干扰（CRISPRI）对 瘦身 物种中特定基因沉默的应用。 瘦素 细胞通过与表达 dCas9（催化"死亡"Cas9） 的质粒结合而发生转化，以及单导RNA （sgRNA），负责与所需的基因组目标进行碱基配对。提出了验证基因沉默的方法。

Tags

遗传学， 第 174 期， 瘦肉精 ， 瘦素， 突变， CRISPR 干扰， 基因沉默， 结合
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