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JoVE Journal
Biochemistry

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使用全内反射荧光(TIRF)显微镜对脂质双层中的横向迁移率和离子通道活性进行单分子成像
 
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使用全内反射荧光(TIRF)显微镜对脂质双层中的横向迁移率和离子通道活性进行单分子成像

Article DOI: 10.3791/64970-v 08:55 min February 17th, 2023
February 17th, 2023

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Summary

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Click here for the English version.

该协议描述了如何使用TIRF显微镜来跟踪单个离子通道并确定它们在支持的脂质膜中的活性,从而定义侧向膜运动和通道功能之间的相互作用。它描述了如何制备膜、记录数据并分析结果。

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生物化学,第192期,
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