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Journal / Biochemistry / Single-Molecule Imaging van Laterale Mobiliteit…
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Biochemistry

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JoVE Journal Biochemistry
Single-Molecule Imaging van Laterale Mobiliteit en Ion Channel Activity in Lipid Bilayers met behulp van Total Internal Reflection Fluorescence (TIRF) Microscopie
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Single-Molecule Imaging van Laterale Mobiliteit en Ion Channel Activity in Lipid Bilayers met behulp van Total Internal Reflection Fluorescence (TIRF) Microscopie

Article DOI: 10.3791/64970-v • 08:55 min • February 17th, 2023
February 17th, 2023 •
1, 1
1Biophysics Department, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart

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Dit protocol beschrijft hoe TIRF-microscopie kan worden gebruikt om individuele ionkanalen te volgen en hun activiteit in ondersteunde lipidemembranen te bepalen, waardoor het samenspel tussen laterale membraanbeweging en kanaalfunctie wordt gedefinieerd. Het beschrijft hoe de membranen moeten worden voorbereid, de gegevens moeten worden vastgelegd en de resultaten moeten worden geanalyseerd.

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Biochemie Nummer 192
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