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Genetics
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JoVE Journal Genetics
Daglige overførsler, arkivering af populationer og måling af kondition i det langsigtede evolutionseksperiment med Escherichia coli
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Daglige overførsler, arkivering af populationer og måling af kondition i det langsigtede evolutionseksperiment med Escherichia coli

Article DOI: 10.3791/65342-v • 15:00 min • August 18th, 2023
August 18th, 2023 •
1,2, 2,3, 2,4, 1, 1, 2,3, 2,5
1Department of Molecular Biosciences, The University of Texas at Austin, 2BEACON Center for the Study of Evolution in Action, Michigan State University, 3Department of Microbiology and Molecular Genetics, Michigan State University, 4Department of Integrative Biology, Michigan State University, 5Biological Sciences Program, Michigan State University

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Denne protokol beskriver, hvordan man opretholder Escherichia coli Long-Term Evolution Experiment (LTEE) ved at udføre sine daglige overførsler og periodiske frysninger, og hvordan man udfører konkurrenceanalyser for at måle fitnessforbedringer i udviklede bakterier. Disse procedurer kan tjene som en skabelon for forskere, der starter deres egne mikrobielle evolutionseksperimenter.

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Genetik nr. 198
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