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Journal / Genetics / Daily Transfers, Archiving Populations, and…
JoVE Journal
Genetics
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JoVE Journal Genetics
Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with Escherichia coli (대장균을 사용한 장기 진화 실험에서의 일일 이동, 인구 보관 및 적합성 측정)
Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
Journal (Genetics)
Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
Journal (Genetics)
Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
 
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Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with Escherichia coli (대장균을 사용한 장기 진화 실험에서의 일일 이동, 인구 보관 및 적합성 측정)

Article DOI: 10.3791/65342-v • 15:00 min • August 18th, 2023
August 18th, 2023 •
1,2, 2,3, 2,4, 1, 1, 2,3, 2,5
1Department of Molecular Biosciences, The University of Texas at Austin, 2BEACON Center for the Study of Evolution in Action, Michigan State University, 3Department of Microbiology and Molecular Genetics, Michigan State University, 4Department of Integrative Biology, Michigan State University, 5Biological Sciences Program, Michigan State University

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이 프로토콜은 일일 이동 및 주기적인 동결을 수행하여 대장균 LTEE(Long-Term Evolution Experiment)를 유지하는 방법과 진화된 박테리아의 적합성 향상을 측정하기 위해 경쟁 분석을 수행하는 방법을 설명합니다. 이러한 절차는 연구자가 자신의 미생물 진화 실험을 시작하기 위한 템플릿 역할을 할 수 있습니다.

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