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Journal / Genetics / Double immunofluorescence de γH2AX et 53BP1 dans…
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JoVE Journal Genetics
Double immunofluorescence de γH2AX et 53BP1 dans les lymphocytes périphériques humains
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Double immunofluorescence de γH2AX et 53BP1 dans les lymphocytes périphériques humains

Article DOI: 10.3791/65472-v • 05:34 min • July 14th, 2023
July 14th, 2023 •
1, 1,2, 1, 1
1Unità di Genetica, Dipartimento di Biologia, University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico

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Ce protocole présente une méthode pour évaluer la formation et la réparation des cassures double brin d’ADN par la détection simultanée des foyers γH2AX et 53BP1 dans les noyaux interphasiques des lymphocytes périphériques humains traités à la bléomycine.

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Ce mois-ci dans JoVE numéro 197
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