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Journal / Genetics / ヒト末梢リンパ球におけるγH2AXと53BP1の二重免疫蛍光法…
JoVE Journal
Genetics
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JoVE Journal Genetics
ヒト末梢リンパ球におけるγH2AXと53BP1の二重免疫蛍光法
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ヒト末梢リンパ球におけるγH2AXと53BP1の二重免疫蛍光法

Article DOI: 10.3791/65472-v • 05:34 min • July 14th, 2023
July 14th, 2023 •
1, 1,2, 1, 1
1Unità di Genetica, Dipartimento di Biologia, University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico

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このプロトコルは、ブレオマイシン処理されたヒト末梢リンパ球の間期核におけるγH2AXおよび53BP1病巣の同時検出を通じて、DNA二本鎖切断の形成および修復を評価する方法を提示する。

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今月のJoVE、第197号、
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