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Chapters
- 00:00Title
- 00:53Introduction
- 01:15Loading Cells with Fura-2 Calcium Indicator Dye
- 05:02Calcium Imaging of Cells
- 09:27Defining and Analyzing Regions of Interest (ROI)
- 10:29Representative Results/Outcome
- 11:30Conclusion
Calcium signals play a key role in many cellular processes including gene expression, survival and differentiation. Here we demonstrate how to perform calcium imaging using Fura-2 AM. Calcium imaging is a valuable tool to study the regulation of intracellular calcium in real time and its regulation of signaling cascades.
