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Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
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1USGS Western Ecological Research Center, 2Wildlife Health Center,University of California, Davis, 3Department of Population Health and Reproduction,University of California, Davis, 4Department of Veterinary and Biomedical Sciences,University of Minnesota , 5Science Applications International Corporation
Chapters
- 00:05Title
- 01:37Shorebird Capture using Mist Nets
- 03:35Oropharyngeal Swab Sampling
- 04:34Sample Preparation
- 06:23Operating the Ruggedized Advanced Pathogen Identification Device (R.A.P.I.D.)
- 07:46Representative RAPID Analysis Results
- 08:22Conclusion
This study describes diagnosis of avian influenza in wild birds using a portable rRT-PCR system. The method takes advantage of freeze-dried reagents to screen wild birds in a non-laboratory setting, typical of an outbreak scenario. Use of molecular tools provides accurate and sensitive alternatives for rapid diagnosis.
