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Chapters
- 00:05Title
- 01:14Production of HIV-1 Particles
- 03:04Concentration of HIV-1 Virions by Ultracentrifugation
- 04:30Sucrose Gradient Centrifugation to Isolate Cores
- 06:36Kinetic Assay of HIV-1 Uncoating
- 10:21Conclusion
Uncoating is an essential step in the early phase of the HIV-1 life cycle and is defined as the disassembly of the capsid shell and the release of the viral ribonucleoprotein complex (vRNP). Here, we demonstrate techniques for isolating intact cores from HIV-1 virions and for quantifying their uncoating in vitro.
Tags
In Vitro UncoatingHIV-1 CoresRetrovirusesCapsidLipid EnvelopeLentivirusesCA ProteinHexagon LatticePentamersCone StructureViral Ribonucleoprotein ComplexFusionTarget Cell MembraneCytoplasmDisassemblySoluble CAUncoating ProcessIntracellular LocationTimingAmino-acid SubstitutionsStability Of The CapsidHIV-1 InfectionAssaysQuantitative MethodIsolation Of CoresSedimentationConcentrated VirionsDetergent LayerSucrose Gradient
